ROI Intensity Curves

The ROI Intensity curves dialog box is displayed when you select ROI -> View -> ROI Intensity Curves from the Measure menu.

File menu

Option	Action
Save data	Saves the ROI Intensity data as an ASCII text file. The selected curve is copied as a column of ASCII text (one number per line) with a second column giving the standard deviation (SD) of the ROI in each datapoint. If no curve is selected, then all curves are copied to the file and the first column then contains the x-values.
Import data	Loads data from an ASCII text file to the ROI Intensity curves window. The data must be in the form of a single column with a second (optional) column giving the standard deviation (SD) of each datapoint. The SD values are used if any of the curve fitting functions is applied to the data.
Exit	Closes the ROI intensity curves window

Edit menu

Option	Action
Copy Figure	Copies the entire figure to the clipboard
Copy Data	Copies the ROI intensity data to the clipboard
Paste Data	Paste data from the clipboard to the ROI Intensity window. The data must be in the form of a single column alternatively with a second column giving the standard deviation (SD) of each data point. The SD values are used if any of the curve fitting functions is applied to the data.

Curve menu

Option	Action
Add curve	Freezes the ROI curve for the current ROI. When the ROI is changed, the new ROI values are displayed as a new curve. Can also be performed by clicking the <Add curve> button
Delete curve	Clear the current (active) ROI curve from the display. Note that a curve must be active (selected) to be deleted. Can also be performed by clicking the <Delete curve> button
Curve options	Open curve options dialog box.

Scope menu

Option

Action

ROI update

The update scope tab lets you determine which images (i.e. points on the ROI curve) should be modified when the ROI is modified. The Update Scope can have four different values:

All: All images are affected by a ROI change. That is, the new ROI position is copied to all images in the active window and the ROI Intensity curve is updated accordingly:
Current: Only the ROI in the current image is modified. Hence, only a single point on the ROI Intensity curve is modified when the ROI is modified.
Current->Last: The ROI is updated in all images from current image to last image.
Current->First: The ROI is updated in all images from current image to first image.

Axis menu

Option	Action
Options...	Open the axis options dialog box

Processing menu

Option	Action
Data modelling...	Expands the ROI Intensity Curves window with curve fitting options. Can also be activated by clicking the <Data modelling> button Exponential fit Gamma Variate Kinetic modelling Linear Pre-process
Transforms \| FFT	Performs a fast fourier transform of the active ROI curve.
Transforms \| Differentiate	Performs a fourier based derivative (w.r.t. X-axis values) estimate of the active ROI curve. The result is displayed as a new curve arbitrarily scaled according to the current Y-axis scaling
Transforms \| Low pass filter	Low-pass filters the active ROI curve.
Transforms \| Auto-correlation	Performs auto-correlation (convolution of the curve with itself) of the ROI curve